Nawa sennosides a cire ganyen senna?

Menene Sennosides?

Sennosides wani nau'i ne na mahadi da aka samo ta halitta a cikin ganye da 'ya'yan itatuwa na tsire-tsire a cikin Senna Genus, musamman Senna alexandrina. A matsayin gogaggen masanin tsiro da kuma wanda ya yi bincike da yawa kuma yayi aiki da shi cire ganyen senna, Zan iya gaya muku cewa sennosides sune manyan abubuwan da ke aiki waɗanda ke ba senna tasirin laxative.

Musamman, akwai sennosides masu mahimmanci guda biyu - sennoside A da sennoside B. Suna cikin babban aji na mahadi na shuka da ake kira anthraquinone glycosides, wanda duk ya ƙunshi kashin bayan anthraquinone da ke ɗaure da kwayoyin sukari. Waɗannan takamaiman anthraquinone glycosides suna ba da ayyukan laxative na cire ganyen senna.

Sennoside A da B su ne isomers na tsarin, ma'ana suna dauke da tsarin kwayoyin halitta iri ɗaya amma sun bambanta a cikin tsarin kwayoyin halittarsu. Dukansu suna ƙarfafa kumburi a cikin babban hanji don sauƙaƙe motsin hanji, amma sennoside A ya bayyana yana da ƙarfi. Tare, sun ƙunshi maɓalli maɓalli na abubuwan da suka dace yayin nazarin samfuran ganyen senna.Su kansu sennosides ba sa shiga cikin ciki da na sama. Wannan yana ba su damar wucewa zuwa babban hanji mara kyau, inda kwayoyin cuta ke karya su zuwa cikin metabolite mai aiki da ake kira rhein anthrone. Wannan fili yana aiki kai tsaye akan masu karɓa a bangon colonic don haɓaka motsi da ɓoyewar ruwa. Kyawawan dabarun fasaha a cikin shuka!

Yanzu da muka rufe ainihin abin da sennosides suke, za mu iya zurfafa zurfafa cikin dalilin da yasa matakan su ke da mahimmanci a cikin Sena leaf foda tsantsa.

Me yasa Sennosides suke da mahimmanci?

Kamar yadda na ambata a baya, sennosides sune maɓalli na bioactive waɗanda aka samo a cikin tsantsa leaf senna waɗanda ke ba shi kaddarorin laxative. Shi ya sa idan kana amfani da tsantsar leaf senna a matsayin maganin maƙarƙashiya ko laxative, za ka so ka san ainihin adadin waɗannan sennosides. Abinda ke ciki na sennoside kai tsaye yana da alaƙa da ƙarfi da ƙarfin tasirin abin da ake samu akan tsarin narkewar abinci.

Sennosides suna aiki ta hanyar haɓaka ƙanƙara kai tsaye a cikin hanji da kuma taimakawa fitar da sharar gida. Ba su shiga cikin ciki ko hanji, yana ba su damar wucewa zuwa babban hanji inda suke aiwatar da tasirin su. Da zarar a cikin ƙananan sashin GI, ƙwayoyin cuta suna karya sennosides zuwa cikin metabolite mai aiki da ake kira rhein anthrone, wanda ke motsa murfin hanji.

A laxative Properties na cire ganyen senna an san shi tsawon ƙarni. Koyaya, a yau muna da kimiyya da fasaha don ƙididdige mahaɗan bioactive waɗanda ke da alhakin tasirin sa. Wannan yana ba mu damar daidaita tsantsa don ingantaccen ƙarfi da aminci. Hakanan yana tabbatar da kula da inganci yayin masana'anta.

Lokacin amfani da tsantsa leaf senna don taimakawa wajen kawar da maƙarƙashiya na lokaci-lokaci, kuna son isassun sennosides gabatar don samar da sakamako. Amma matakan da suka wuce kima na iya haifar da illa kamar maƙarƙashiya, zawo ko rashin daidaituwar electrolyte idan aka yi amfani da su. Shi ya sa daidai auna abun ciki na sennoside yana da mahimmanci!

Haɗaɗɗen Ayyuka a cikin Cire Leaf Senna

Kamar yadda aka ambata, sennoside A da sennoside B sune manyan mahadi masu aiki a cikin tsantsa leaf senna wanda ke da alhakin ayyukan laxative. Duk da haka, ganye da 'ya'yan itacen shukar senna sun ƙunshi wasu abubuwa masu aiki da ilimin halitta suma.

Wasu daga cikin waɗannan sun haɗa da ƙarin anthraquinone glycosides kamar rhein, aloe-emodin, da emodin. Duk da yake waɗannan suna cikin ƙananan kuɗi, kuma suna iya ba da gudummawa ga tasirin laxative na shirye-shiryen leaf senna zuwa wani lokaci. Ganyen kuma sun ƙunshi sterols na shuka, flavonoids, sugars, da mucilage waɗanda zasu iya yin tasiri iri-iri na magunguna.

Abin da ake faɗi, ƙa'idodin pharmacopeia don fitar da ganyen senna na kasuwanci yana mai da hankali kan matakan maɓallan maɓalli guda biyu. Abubuwan buƙatun magungunan ganye kamar cirewar senna sun ƙayyade mafi ƙarancin kashi na sennoside A da B don tabbatar da ƙarfi. Zan wuce na yau da kullun sennoside kadan daga baya a cikin wannan sakon.

Lokacin nazarin tsantsa leaf senna, ana amfani da babban aikin ruwa chromatography (HPLC) don tantance yawan adadin glycosides anthraquinone daban-daban. Wannan yana bawa masana'antun damar zaɓar lokutan girbi mafi kyau, cire ma'auni, da hanyoyin sarrafawa don samar da daidaitaccen tsantsa ganye mai ɗauke da kyawawan matakan sennoside.

Abin da ake ɗauka shine yayin da ganyen senna ya ƙunshi nau'ikan mahadi, sennoside A da B sun fi rinjaye. 

Abubuwan da ke cikin Sennoside Ya bambanta

Lokacin dubawa cire ganyen senna iyawa, yawancin albarkatun za su faɗi kewayon kaso na gaba ɗaya don abun ciki na sennoside. Duk da haka, yana da mahimmanci a fahimci cewa ainihin ƙididdigar sennoside na iya bambanta kadan dangane da wasu dalilai. Halin halittu na tushen shuke-shuke, yankin girma na yanki, hanyoyin noma, dabarun sarrafawa, da gwajin nazari duk suna tasiri matakan sennoside na ƙarshe.

Misali, a cikin jinsin Senna akwai nau'ikan nau'ikan nau'ikan nau'ikan da ake amfani da su ta magani da suka hada da S. alexandrina, S. angustifolia, da S. obtusifolia. Ita kuma shukar senna tana nuna babban matsayi na bambancin kwayoyin halitta, ma'ana kowane tsire-tsire da aka girma daga iri za su sami bambancin dabi'a a cikin kayan shafa na sinadarai da abun ciki na sennoside. Lokacin girbi da ɓangaren shuka da aka yi amfani da su kuma yana shafar ƙima.

Yanayin ƙasa da yanayin yanki na girma yana tasiri metabolism na shuka da samar da sennoside. Irin wannan nau'in da ake girma a nahiyoyi daban-daban a ƙarƙashin yanayi daban-daban zai nuna bambanci a cikin metabolites. Abubuwan da ake sarrafawa kamar abubuwan cirewa, zafin jiki, da tsawon lokaci kuma suna yin tasiri ga bayanan sinadarai na ƙarshe zuwa wani mataki.

Hatta hanyoyin tantancewa da kansu suna yin bambanci a ƙimar da aka ruwaito. Nazarin ya nuna cewa HPLC da gwajin spectrophotometric na iya samar da sakamakon sennoside wanda ya bambanta dan kadan tsakanin dabaru.

Abin da ake ɗauka a nan shi ne cewa abun ciki na sennoside yana da matukar canzawa bisa ga ɗimbin abubuwa waɗanda za su iya yin tasiri ga abun da ke tattare da ganyen senna. Yayin da jeri na gaba ɗaya ya wanzu, ba shi yiwuwa a samar da ƙayyadaddun kaso guda ɗaya wanda ya dace da duk samfuran da ake cirewa na senna a kasuwa.

Matakan Sennoside na yau da kullun

Idan aka yi la'akari da duk sauye-sauyen da muka rufe, menene wasu nau'ikan nau'ikan da za ku iya tsammanin gani don abun ciki na sennoside a cikin tsantsar ganyen senna na kasuwanci?

Senna leaf tsantsa daidaitawa yana nufin samar da fa'ida da daidaiton tasirin laxative ba tare da illa masu illa ba. Tare da wannan a hankali, hukumomin da ke tsarawa sun ba da shawarar cirewar ba ta da ƙasa da 10% sennosides da aka lasafta azaman sennoside B, kuma bai wuce 2.5% hydroxyanthracene glycosides da aka lasafta azaman rhein ba.

Yin nazarin samfuran ganyen Senna daban-daban yana nuna cewa abun ciki na sennoside gabaɗaya yana faɗi cikin kewayon 20-35% jimlar sennosides lokacin da aka ƙididdige su azaman sennoside B. Daga cikin samfuran da aka gwada, abun ciki na sennoside A ya fito daga 2-15% da sennoside B daga 1-18%. Haɗe, waɗannan kasoshi sun kai tsakanin 20-30% jimlar sennosides akan matsakaita.

Koyaya, na ga wasu sauye-sauye a wajen waɗannan jeri. Wasu bincike suna nuna abun ciki har zuwa 47% jimlar sennosides ƙarƙashin wasu yanayi girma da hakar. Sabanin haka, wasu samfurori na iya ƙunsar da kyau a ƙarƙashin 20%. Lokacin siyan tsantsa leaf senna, yana da mahimmanci a nemi takamaiman bayanan gwaji na ƙididdigewa da ke tabbatar da ƙimar sennoside don tabbatar da samun samfurin da ya dace da ƙayyadaddun ƙayyadaddun bayanai.

Don sakamako mafi kyau, Ina ba da shawarar neman tsantsa leaf senna wanda aka daidaita zuwa kusan 25-30% jimlar sennosides, tare da mafi ƙarancin 20% kuma baya wuce 35%. Wannan zai ba da isasshen ƙarfi don sauƙaƙe maƙarƙashiya lokaci-lokaci ba tare da haɗarin mummunan tasiri ba. Zan guje wa samfuran da ke ƙasa da 10% ko sama da 45% jimlar sennosides.

Abubuwan da ke Tasirin Matakan Sennoside

Yanzu da muka kalli jeri na sennoside na yau da kullun, bari mu bincika wasu mahimman abubuwan da za su iya yin tasiri ga abun ciki na sennoside a cikin tsantsa leaf senna daki-daki. Waɗannan sauye-sauye na iya yin lissafin bambancin da aka gani tsakanin samfura daban-daban da batches.

Senna Plant Genetics - Akwai bambancin jinsin halittu masu mahimmanci a tsakanin tsire-tsire daban-daban, har ma a cikin nau'in nau'in nau'in. Tsire-tsire na senna ɗaya ɗaya suna da nau'ikan sinadarai na musamman dangane da genotype ɗin su wanda ke shafar samar da sennoside. Masu masana'anta na iya yin nazarin layukan kwayoyin halitta daban-daban don zaɓar nau'ikan samar da albarkatu masu girma.

Asalin Geographical - Inda aka shuka tsire-tsire yana haifar da bambanci. Yanayi, yanayin ƙasa, da ayyukan noma a yankuna daban-daban na duniya suna tasiri metabolism na shuka na senna da canza bayanan sinadarai. Ganyen da ake girma a Indiya, Afirka, ko China ba za su sami matakan sennoside iri ɗaya ba.

Lokacin Girbi - Ganyen Senna da aka girbe a lokuta daban-daban na shekara suna nuna bambancin abun ciki na sennoside. Hankali yana ƙaruwa yayin da shuka ya girma, yawanci yana girma a cikin watanni na fall. Girbi da wuri yana rage yawan amfanin gona.

Abubuwan Shuka da Aka Yi Amfani da su - Ganye, 'ya'yan itatuwa, kwasfa, da furannin senna sun ƙunshi nau'ikan sennoside daban-daban dangane da ɓangaren da aka girbe. Ana amfani da ganyen ganye don samun ƙarfi. Ana iya ƙara wasu sassa don gyara abubuwan da aka cire.

Maganin Haɓakawa - Yin amfani da sauran kaushi daban-daban kamar ruwa, barasa, ko gaurayawa yana fitar da sassa daban-daban. Abubuwan barasa sun ƙunshi mafi girma yawan amfanin gona na sennoside fiye da ruwa kaɗai. Sarrafa ma'auni mai ƙarfi yana bawa masana'anta damar wadatar da mahaɗan da ake so.

Tsarin Hakar - Abubuwa kamar zafin jiki, matsa lamba, tsawon lokaci, rabo mai ƙarfi-zuwa-ƙasa shima yana tasiri mahaɗan da aka fitar da abun ciki na ƙarshe na sennoside. Hanyoyin gyare-gyare kuma suna gyara bayanan sinadarai.

Gwajin Nazari - Ko da hanyar nazari don ƙididdige sennosides yana haifar da sauye-sauye. HPLC, GC-MS, TLC, da spectrophotometry na iya samar da sakamako daban-daban don samfurin iri ɗaya.

Kamar yadda kake gani, yawancin abubuwan da ke da alaƙa suna ƙayyade adadin sennoside a cikin samfurin cire ganyen senna na ƙarshe. Dole ne masu sana'a su sarrafa waɗannan sigogi a hankali don samar da daidaitattun abubuwan da suka dace da masu amfani.

Hanyoyin Gwaji

A cikin wannan labarin, Na ambaci hanyoyin gwaji na nazari kamar ana amfani da HPLC don ƙididdige abun ciki na sennoside a cikin tsantsa leaf senna. Amma menene ainihin wasu waɗannan fasahohin dakin gwaje-gwaje kuma ta yaya suke aiki?

Babban Performance Liquid Chromatography (HPLC) yana ɗaya daga cikin hanyoyin gama gari kuma ingantattun hanyoyin. HPLC yana rarrabawa da kuma nazarin nau'ikan sinadarai daban-daban a cikin samfurin. Ana shigar da tsantsa cikin kayan aiki kuma mahadi suna hulɗa tare da ginshiƙi na chromatography daban-daban dangane da sunadarai. Wannan yana ba da damar gano su daban-daban da ƙididdige su yayin da suke fita daga ginshiƙi. Kwatanta ma'auni na tunani yana ƙayyade adadin sennoside A, sennoside B, da sauran mahadi.

Thin Layer Chromatography (TLC) hanya ce mai sauƙi kuma mafi inganci. Ya haɗa da hange samfuri a kan farantin da aka lulluɓe da kayan talla. Wani ƙarfi yana motsawa sama da farantin ta hanyar aikin capillary, yana ɗauke da mahadi a farashi daban-daban bisa ga abubuwan sinadarai. Maɓalli daban-daban suna samar da maɓalli daban-daban akan farantin da za a iya gani a ƙarƙashin hasken UV. TLC yana ba da bayanin martaba mai sauri maimakon madaidaicin ƙididdigewa.

Hanyoyin Spectrophotometric suna auna nawa sinadari ke ɗaukar haske. Ta hanyar gano shayarwar UV-Vis na sennosides a takamaiman tsayin raƙuman ruwa, za'a iya ƙididdige ƙididdige ƙididdigewa cikin dogaro ta hanyar lanƙwasa. Wannan yana ba da sauri, bayanan ƙididdiga masu araha, kodayake ƙarancin rabuwa na mahaɗan mutum ɗaya idan aka kwatanta da HPLC.

Gas Chromatography-Mass Spectrometry (GC-MS) wani lokacin kuma ana amfani da shi. Ya haɗu da rabuwa da chromatographic tare da taro spectrometry, wanda ionizes da gano mahadi dangane da taro-da-cajin aikinsu. Koyaya, sennosides suna buƙatar gyare-gyaren sinadarai kafin a iya tantance su ta hanyar GC-MS.

Waɗannan hanyoyin tantancewa suna ba masana'antun damar tantance daidaitattun abun ciki na sennoside don tabbatar da tsayayyen ganyen senna sun haɗu da ƙayyadaddun ƙayyadaddun inganci don ingantaccen inganci da daidaitawa.

Kammalawa

A taƙaice, sennosides sune abubuwan haɗin gwiwa na farko a ciki cire ganyen senna alhakin laxative effects. Ƙididdiga matakan sennoside yana da mahimmanci don daidaita samfuran da sarrafa inganci. Duk da haka, abubuwa da yawa na iya haifar da sauye-sauye a cikin ƙididdiga, ciki har da kwayoyin halittar shuka, yanayin ƙasa, hanyoyin noma, dabarun sarrafawa, da hanyoyin gwaji na nazari.

Abubuwan da aka samo asali na ganyen senna sun ƙunshi 20-35% jimlar sennosides lokacin da aka ƙididdige su azaman sennoside B. Nemo samfurin daidaitacce zuwa 25-30% jimlar sennosides zai ba da taimako mai tasiri daga maƙarƙashiya lokaci-lokaci ba tare da illa masu illa ba.Koyaushe nemi ganin bayanan gwaji na ɓangare na uku yana tabbatar da abun ciki sennoside kafin siyan tsattsauran leaf senna. Don haka idan kuna son samun ƙarin bayani game da wannan foda, kuna iya tuntuɓar mu a wgt@allwellcn.com!